美国 Bio-rad转基因仪基因枪系统Helios

Helio基因枪是一种方便的手提式设备，可对细胞进行快速直接的原位基因转移。该系统通过可调节的氦气脉冲，来带动位于 小塑料管内壁处预包有DNA、RNA 或其它生物材料的金粉颗粒，将其直接打入细胞内部。使用样品管制备站可很容易地制备管内 “子弹”。
 

颗粒轰击用于体外和体内基因转移的优点
* 简单、快速、功能灵活的基因传送，各种细胞类型通用
* 有助于瞬时表达和稳定表达
* 只需少量的DNA 和细胞
* 无需载体DNA
* 能进行多质粒共同导入
* 输送大片段DNA
* 胞内目标基因可导入多个细胞
* 用于体内和体外转化
* 没有无关的基因或蛋白被转导

The Helio gene gun is a convenient portable device for rapid and direct in-situ gene transfer of cells.The system USES an adjustable pulse of helium gas to drive gold particles of DNA, RNA or other biomaterials prepacked on the inside of a small plastic tube directly into a cell.The in-tube "bullet" can be easily prepared using the sample control station.

The advantages of particle bombardment for gene transfer in vitro and in vivo

* simple, fast, functional gene transfer across all cell types

* contributes to transient and stable expression

* only small amounts of DNA and cells are needed

* no carrier DNA is required

* multiple plasmids can be imported together

* transport large fragments of DNA

* target genes can be introduced into multiple cells

* for in vivo and in vitro transformation

* no unrelated genes or proteins are transduced